Raw data for the manuscript “Activation of mTOR pathway by human cytomegalovirus promoting host ribosomal protein expression by coordinated transcriptional and translational controls”

This dataset contains the raw and processed data supporting the manuscript <i>“Activation of mTOR pathway by human cytomegalovirus promoting host ribosomal protein expression by coordinated transcriptional and translational controls.”</i>Data are organized by figure numbers (Fig1–Fig5 and Supplementary Figures) and include:<b>Transcriptomics</b>: CAGE-seq and RNA-seq analyses of transcription start site (TSS) usage and differential expression.<b>Proteomics</b>: Label-free quantitative MS (LFQ/MS) and targeted PRM validation.<b>Validation assays</b>: PCR array, 5’RACE sequencing, PAGE results.<b>Functional studies</b>: Reporter plasmid assays, Western blot quantifications, fluorescence microscopy, and viral titration experiments.These datasets illustrate how HCMV infection drives alternative TSS switching in ribosomal protein genes (RPL4, RPS11, RPS23, RPS24), enhances 5’TOP motif isoform abundance, and activates mTOR-dependent translational regulation to promote viral replication.

本数据集包含支持下述研究手稿的原始与处理后数据，手稿标题为《"人类巨细胞病毒激活mTOR（哺乳动物雷帕霉素靶蛋白）通路，通过协同转录与翻译调控促进宿主核糖体蛋白表达"》。数据按图号分类整理（Fig1至Fig5及补充图），具体包含以下内容：
1. 转录组学（Transcriptomics）：针对转录起始位点（Transcription Start Site, TSS）使用情况与差异表达的CAGE-seq与RNA-seq分析。
2. 蛋白质组学（Proteomics）：无标记定量质谱（Label-free quantitative MS, LFQ/MS）与靶向PRM验证实验。
3. 验证实验（Validation assays）：PCR芯片、5’RACE测序、聚丙烯酰胺凝胶电泳（Polyacrylamide Gel Electrophoresis, PAGE）结果。
4. 功能研究（Functional studies）：报告质粒实验、蛋白质免疫印迹定量分析、荧光显微镜观测及病毒滴度测定实验。

本数据集阐明了人类巨细胞病毒（Human Cytomegalovirus, HCMV）感染如何驱动核糖体蛋白基因（RPL4、RPS11、RPS23、RPS24）发生可变转录起始位点切换，提升5’TOP基序亚型的丰度，并激活mTOR依赖性翻译调控以促进病毒复制。