mRNA reduction of the downstream targets of PITX3 in miak mice at embryonic stages.

The relative levels of Prox1, Foxe3, and Mip mRNA in the eye of wild-type (+/+) and miak/miak mice at E11.5, E12.5 and E14.5. The mRNA expression levels were measured by real-time RT-PCR analysis using specific primer sets (Table S1) for each gene. The values shown in each graph indicate the mean relative expression levels and the SDs of triplicate eye mRNAs. The expression levels in wild-type mice at E11.5 were assigned an arbitrary value of 1 for comparative purposes. **P0.01; ***P0.001.

本数据集检测了野生型（wild-type，基因型为+/+）及miak/miak纯合突变小鼠在胚胎发育第11.5天（E11.5）、第12.5天（E12.5）与第14.5天（E14.5）的眼球组织中Prox1、Foxe3及Mip mRNA的相对表达水平。mRNA表达水平通过实时逆转录聚合酶链反应（real-time RT-PCR）分析测得，实验针对各基因使用了特异性引物组（附表S1）。各图表中展示的数值为三次重复眼球mRNA样本的相对表达均值与标准差（SD）。为便于组间比较，将野生型小鼠在E11.5时的mRNA表达水平设为任意参照值1。**P<0.01；***P<0.001.