Gene signature of the post-Chernobyl papillary thyroid cancer

Thyroid gland is among the most sensitive organs to ionizing radiation. Whether low-dose radiation-induced papillary thyroid cancer (PTC) differs from sporadic PTC is yet unknown. We used microarrays to identify gene signature of radiation-induced papillary thyroid carcinomas To identify molecular differences between radiation-induced (Exposed to Chernobyl Radiation, ECR) and sporadic PTC, we investigated 65 childhood/young adult PTC samples using DNA microarray (Affymetrix, Human Genome U133 2.0 Plus). The PTC samples were from patients born either before (33 ECR cases) or at least 9 months after (32 non-ECR cases) the Chernobyl catastrophe. Multofactoral analyses were performed in order to define some additional factors that could have impact on the gene expression profile. Morover the microarray data were validated with the QPCR reaction and exon arrays.

甲状腺是对电离辐射最为敏感的器官之一。低剂量辐射诱发的乳头状甲状腺癌（papillary thyroid cancer, PTC）与散发性PTC是否存在差异，目前仍未明确。为筛选辐射诱发乳头状甲状腺癌的基因特征，并明确辐射诱发（曾暴露于切尔诺贝利辐射，ECR）与散发性PTC的分子差异，我们采用DNA微阵列（Affymetrix人类基因组U133 2.0 Plus芯片）对65例儿童/青年PTC样本进行了分析。这些PTC样本的供体患者均出生于切尔诺贝利核事故发生前（33例ECR病例），或核事故发生至少9个月后（32例非ECR病例）。为确定可能对基因表达谱产生影响的其他潜在因素，我们开展了多因素分析。此外，本研究还通过QPCR反应与外显子芯片对微阵列数据进行了验证。