Microglia care for neurons through tunneling nanotubes [Dataset 2 Microglia mRNA-seq]. Microglia care for neurons through tunneling nanotubes [Dataset 2 Microglia mRNA-seq]

We report the transfer of alpha-s-ynuclein aggregates from neurons to microglia and examined transcriptomic changes following protein extraction. By obtaining RNA samples, we performed differential expression analysis and generated gene ontology enrichment and network analysis. Overall design: mRNA sequencing of Microglia from mice cocultured in the presence of murine neurons treated with and without aSyn

本研究报道了α-突触核蛋白（α-synuclein）聚集物从神经元向小胶质细胞（microglia）的转运过程，并检测了蛋白质提取后的转录组学变化。通过获取RNA样本，我们开展了差异表达分析，并完成了基因本体（Gene Ontology）富集分析与基因网络分析。
整体实验设计：对与经aSyn处理或未处理的小鼠神经元共培养的小鼠小胶质细胞进行mRNA测序（mRNA sequencing）。