Profiling the immune cell gene signature of in vivo model of IBD

Identification of genes differentially expressed in inflamed and intact colon samples of TNBS treated in vivo rat model of Inflammatory Bowel Disease To investigate the transcriptional changes between inflamed and phenotypically normal or uninflamed tissue regions along the colons, male Wistar rats were randomly divided into two groups: first group served as control (n=2; vehicle-treated hence non-colitis induced) and the second group was induced by 2,4,6-trinitrobenzene sulphonic acid (TNBS) (n=6; colitis-induced). TNBS was intracolonically administered in a volume of 0.25 ml, via an 8 cm long plastic catheter under transient ether anaesthesia. 72 hours after the treatment all animals were sacrificed, ~8cm of distal colons were removed, opened longitudinally and gently cleaned of fecal content using ice-cold physiological saline. In the case of the control group, since non-inflamed, samples were taken from random colon sections. In case of colitis-induced animals, samples were taken from inflamed colon region as well as from non-adjacent uninflamed region.

本研究旨在鉴定2,4,6-三硝基苯磺酸（TNBS）诱导的大鼠体内炎症性肠病（Inflammatory Bowel Disease）模型中，炎症结肠与完整结肠样本的差异表达基因。为探究结肠炎症区域与表型正常（未发炎）组织区域间的转录组变化，将雄性Wistar大鼠随机分为两组：第一组为对照组（n=2，仅给予赋形剂处理，未诱导结肠炎）；第二组经TNBS诱导结肠炎（n=6）。实验过程中，通过长度8 cm的塑料导管，在短暂乙醚麻醉下向大鼠结肠内灌注0.25 ml TNBS溶液。造模72小时后处死所有大鼠，截取约8 cm的远端结肠，纵向剖开后用预冷生理盐水轻柔冲洗以清除粪便内容物。对照组大鼠结肠无炎症，故随机截取结肠组织样本；结肠炎诱导组大鼠则分别采集炎症结肠区域以及非毗邻的未发炎结肠区域的组织样本。