Gene expression analysis of NT193 cells with an epithelial (E) and mesenchymal (M) phenotype. Gene expression analysis of NT193 cells with an epithelial (E) and mesenchymal (M) phenotype

Cells have been collected by limited dilution and mRNA from 3 consequtive passages of cells cultured in Dulbecco’s modified Eagle’s medium (DMEM Dutscher L0104-500) containing 4.5 g/L glucose, L-glutamine, sodium pyruvate supplemented with 10% of inactivated fetal bovine serum (FBS, Dutscher S1510-500), penicillin (10 000 U/mL), streptomycin (10 mg/mL) (Dutscher P06-07100) were used for RNA seq analysis.

本研究通过有限稀释法收集细胞，从经添加有4.5 g/L葡萄糖、L-谷氨酰胺、丙酮酸钠，以及10%灭活胎牛血清（FBS, fetal bovine serum, Dutscher S1510-500）、10000 U/mL青霉素与10 mg/mL链霉素（Dutscher P06-07100）的Dulbecco改良Eagle培养基（DMEM, Dulbecco’s modified Eagle’s medium, Dutscher L0104-500）中连续培养3代的细胞样本中提取mRNA，将其用于RNA测序（RNA-seq）分析。