RNA-seq analysis identifies key genes and signaling pathways involved in Hetian sheep sebaceous gland proliferation promoted by androgen.

In this study, 15 adult Hetian ewes were randomly divided into 5 groups. Hetian ewes were injected with 0, 1, 2, 3 and 4 mg/kg testosterone every 3 days, and treated for 42 days. Back skin tissues were collected on the 42nd day, and the effects of androgen treatment on skin gene expression patterns were explored by transcriptomic sequencing technology. Identify the key genes and signaling pathways of androgens affecting lipid metabolism in sheep skin.

本研究将15只成年和田母羊随机分为5组，各组每隔3天分别注射0、1、2、3、4mg/kg的睾酮，持续处理42天。于处理第42天采集背部皮肤组织，采用转录组测序技术探究雄激素处理对皮肤基因表达谱的调控效应，进而鉴定雄激素影响绵羊皮肤脂质代谢的关键基因及信号通路。