beetle metagenome and metaproteome. beetle metagenome and metaproteome

Odontotaenius disjunctus is a wood-feeding beetle that possesses a digestive tract with four main compartments each of which contains well-differentiated microbial populations, suggesting that anatomical properties and separation of these compartments may enhance energy extraction from woody biomass. Here, we demonstrate how spatially segregated microbiome functionality facilitates lignocellulose deconstruction and transformation in the passalid digestive tract.Using metagenome and metaproteome analyses, along with spectroscopic, isotopic and analytical chemistry approaches, we reconstructed the pathways for sequential lignocellulose transformation, the subsequent fermentation of lignocellulose products, and the distribution of key microbial catalysts throughout the passalid digestive tract.

分节扁胫蜣（Odontotaenius disjunctus）是一种食木甲虫，其消化道具有四个主要功能分区，每个分区均定植有高度分化的微生物群落，这提示该消化道的解剖结构特征与分区空间分隔机制，或可提升宿主从木质生物质中获取能量的效率。本研究阐明了空间分隔的微生物组功能如何促进该扁蜣科昆虫消化道内的木质纤维素解构与转化过程。研究结合宏基因组（metagenome）与宏蛋白质组（metaproteome）分析技术，辅以光谱学、同位素分析与化学分析手段，重构了木质纤维素的连续转化通路、木质纤维素降解产物的后续发酵通路，以及关键微生物催化因子在该扁蜣科昆虫消化道内的分布格局。