H3K79Me3.BG3

modENCODE_submission_4197 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of the major histone modifications across the Drosophila melanogaster genome. The modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Cell Line: ML-DmBG3-c2; Tissue: CNS-derived cell-line; Developmental Stage: third instar larval stage; Genotype: y v f mal; Sex: Unknown; NUMBER OF REPLICATES: 6; EXPERIMENTAL FACTORS: Cell Line ML-DmBG3-c2; Antibody H3K79Me3 (target is H3 K79 Me3)

modENCODE_submission_4197 本提交来自Gary Karpen的modENCODE项目。有关modENCODE项目的完整列表，请访问http://www.genome.gov/26524648。
项目目标：本研究旨在确定黑腹果蝇（Drosophila melanogaster）基因组中主要组蛋白修饰的定位位点。所研究的修饰参与DNA复制、基因表达、基因沉默及遗传等核心染色体生物学功能。本研究将采用染色质免疫沉淀（Chromatin ImmunoPrecipitation, ChIP）结合基因组平铺阵列（genomic tiling arrays）的实验方案。初期将使用三种细胞系与两个胚胎阶段的染色质开展定位分析，后续将把部分靶蛋白的分析拓展至另外四种动物组织/发育阶段。有关数据使用条款与条件，请参阅http://www.genome.gov/27528022 及 http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。
实验类型：ChIP-chip（染色质免疫沉淀-芯片）。
生物样本来源：细胞系：ML-DmBG3-c2；组织：中枢神经系统（CNS）来源细胞系；发育阶段：三龄幼虫期；基因型：y v f mal；性别：未知；重复次数：6；实验因素：细胞系ML-DmBG3-c2；抗体：H3K79Me3（靶标为H3 K79 Me3）