GENCODE PCR-Seq Batch XII. GENCODE PCR-Seq Batch XII

As part of the ENCODE consortium the GENCODE project is producing a reference gene set through manual and automated gene prediction. Selected transcript models are verified experimentally by RT-PCR amplification of at least one of their unique splice junctions followed by sequencing. The experiment targets are manually annotated transcripts with novel or putative status, non-pseudogene biotype and unique splice junctions not validated previously. For Batch XII 416 splice junctions from GENCODE15 (released January 2013) were chosen for experimental verification.

作为ENCODE联盟（ENCODE Consortium）的组成部分，GENCODE项目（GENCODE Project）正通过手动与自动基因预测手段构建参考基因集。针对选定的转录本模型，研究人员通过逆转录聚合酶链反应（Reverse Transcription Polymerase Chain Reaction, RT-PCR）扩增其至少一个独特剪接位点（splice junction）并进行测序，以此完成实验验证。本次实验的验证目标为经手动注释的转录本，具体涵盖具有新型或推定状态、非假基因生物型且其独特剪接位点此前未被验证的转录本。在第十二批次（Batch XII）中，研究人员选取了2013年1月发布的GENCODE 15版本（GENCODE15）中的416个剪接位点用于实验验证。