Interferon-γ couples CD8+ T cell avidity and differentiation during infection. Interferon-γ couples CD8+ T cell avidity and differentiation during infection

Expression and relevance of IFNg signaling in CD8 T cells during infection Overall design: Expression profiling by high throughput sequencing. For 24hrs experiment, C57 Bl6 mice were left naive or infected with Listeria-OVA infected 24 hrs . CD3+ CD8+ T cells were isolated by FACS from the spleen and subjected to scRNAseq. For day 9 experiment, C57Bl6 WT mice and CD8-IFNgRKO mice were infected with LM-OVA. CD3+ CD8+ OVA-tetramer+ T cells were isolated by Fluorescence-activated cell sorting (FACS) from the spleen after 9 days and subjected to scRNA and scTCR sequencing.

感染过程中CD8阳性T细胞内γ干扰素（IFNγ）信号通路的表达及其相关性
实验设计概述：本研究通过高通量测序开展表达谱分析。
24小时实验组：将C57BL/6小鼠分为未致敏（naive）对照组与李斯特菌-卵清蛋白（Listeria-OVA）感染组，感染24小时后取材；通过荧光激活细胞分选术（Fluorescence-activated cell sorting, FACS）从脾脏中分离CD3+CD8+T细胞，进行单细胞RNA测序（scRNAseq）。
第9天实验组：使用李斯特菌-卵清蛋白（LM-OVA，为Listeria-OVA的缩写）感染C57BL/6野生型（WT）小鼠与CD8细胞特异性γ干扰素受体敲除（CD8-IFNgRKO）小鼠；感染9天后，通过荧光激活细胞分选术从脾脏中分离CD3+CD8+卵清蛋白四聚体阳性（OVA-tetramer+）T细胞，分别开展单细胞RNA测序与单细胞T细胞受体测序（scTCR sequencing）。