Genome-wide analysis of High glucose-induced gene expression by Ninjurin1 siRNA

Transcriptional profiling of Mouse cavernous primary endothelial cells (MCECs) in comparing normal glucose condition with high glucose condition or Ninjurin1 siRNA transfection condition. Objective was to determine the effect of ninjurin1 on high glucose conditioned cells on global gene expression. Total RNA obtained from MCECs, which were treated with High glucose (HG) condition or (HG+ninjurin1 siRNA). Biological replicates: 1 control replicates, 1 HG replicates, 1 (HG+ninjurin1 siRNA) replicates.

本数据集为小鼠海绵体原代内皮细胞（Mouse cavernous primary endothelial cells, MCECs）的转录组谱分析数据，旨在对比正常葡萄糖培养、高葡萄糖培养以及高葡萄糖联合Ninjurin1小干扰RNA（siRNA）转染三种实验条件下的细胞状态。本研究的目标为明确Ninjurin1对高葡萄糖处理细胞的全局基因表达的影响。实验提取的总RNA来自经以下三种处理的MCECs：正常葡萄糖培养对照组、高葡萄糖（HG）处理组以及高葡萄糖联合Ninjurin1 siRNA转染组。生物学重复设置为：对照组、高葡萄糖组、高葡萄糖联合Ninjurin1 siRNA转染组各1次生物学重复。