Gene expression of L3 and L5 pyramidal neurons in the DLPFC comparing schizophrenia from bipolar major depressive disorders and unaffected subjects.. Homo sapiens

Impairments in certain cognitive processes (e.g., working memory) are typically most pronounced in schizophrenia (SZ), intermediate in bipolar disorder (BP) and least in major depressive disorder (MDD). Given that working memory depends, in part, on neural circuitry that includes pyramidal neurons in layer 3 (L3) and layer 5 (L5) of the dorsolateral prefrontal cortex (DLPFC), we sought to determine if transcriptome alterations in these neurons were shared or distinctive for each diagnosis. Overall design: We collected brain specimens (n=76) with SZ (n=19; 6 had schizoaffective disorder), BP (n=19; all had bipolar 1 disorder) or MDD (n=19) and UC subjects (n=19) which were matched as tetrads for sex and as closely as possible for age. Each subject has 2 technique replicates.

某些认知过程（如工作记忆(working memory)）的损害通常在精神分裂症(SZ)中最为显著，双相障碍(BP)次之，重性抑郁障碍(MDD)最轻。鉴于工作记忆在一定程度上依赖于包含背外侧前额叶皮层(DLPFC)第3层(L3)与第5层(L5)锥体神经元(pyramidal neurons)的神经环路，本研究旨在明确此类神经元的转录组改变在各诊断组别中是共有特征还是具有诊断特异性。总体实验设计：本研究共收集76例脑组织标本，涵盖精神分裂症组（SZ，n=19；其中6例合并分裂情感性障碍）、双相障碍组（BP，n=19；均为Ⅰ型双相障碍）、重性抑郁障碍组（MDD，n=19）及健康对照组（UC，n=19）；所有标本按性别匹配为四配对组，并尽可能匹配年龄。每个受试者均设置2次技术重复。