Pi uptake kinetics of wild type and transgenic plants.

Maize seedlings were cultured in SP nutrient solution for 20 days, then divided into two groups and grown under SP and LP conditions for an additional 25 days. Then all maize plants were transferred to the nutrient solution that contained 50 µM Pi at the start. Pi uptake was measured as the amount of Pi removed from the nutrient solution. Imax, maximum rate of Pi influx; Cmin, minimum concentration of solution below which no further net influx occurred and Km, Michaelis - Menten constant. Values are means ± SD (n = 3).*indicates statistically significant differences between transgenic lines and the wild type line under the same conditions at the 0.05 level, using Student's t-test. WT, wild type; L1, L2 and L3, transgenic lines; SP, sufficient phosphate (nutrient solution containing 1,000 µM KH2PO4); LP, low phosphate (nutrient solution containing 5 µM KH2PO4).

将玉米幼苗置于SP营养液中培养20天，随后分为两组，分别在SP与LP条件下继续培养25天。随后将所有玉米植株转移至起始含有50 μM无机磷（Pi）的营养液中。磷吸收量以营养液中Pi的减少量进行测定。其中，Imax为Pi内流最大速率（maximum rate of Pi influx），Cmin为无法发生净内流的最低溶液浓度（minimum concentration of solution below which no further net influx occurred），Km为米氏常数（Michaelis-Menten constant）。所有数值均以平均值±标准差（standard deviation, SD）表示，每组重复数n=3。*表示在相同条件下，采用学生t检验（Student's t-test）时，转基因株系与野生型株系间存在0.05显著性水平的统计学差异。WT为野生型（wild type），L1、L2与L3为转基因株系；SP为充足磷营养（sufficient phosphate，即含1000 μM KH₂PO₄的营养液），LP为低磷营养（low phosphate，即含5 μM KH₂PO₄的营养液）。