eDNA metabarcoding using Mifish primers at the North Sea

In June 2019, the Thünen Institute conducted the WH429 research cruise with the aim of assessing marine biodiversity through environmental DNA (eDNA) analysis. During the expedition, eDNA samples were collected from various marine locations and subsequently processed for metabarcoding using the MiFish primers, which target a specific region of the mitochondrial 12S rRNA gene in fish. The resulting eDNA sequences were generated on the Illumina MiSeq platform, producing high-quality fastq files. This dataset provides critical metadata, including sequencing reads, quality scores, which are essential for studying the spatial and temporal patterns of fish diversity. The comprehensive eDNA dataset serves as a valuable resource for ecological and conservation research, enabling the identification of fish species and the monitoring of marine ecosystems with high resolution and accuracy.

2019年6月，瑟内嫩研究所（Thünen Institute）开展了WH429号科研航次，旨在通过环境DNA（environmental DNA, eDNA）分析评估海洋生物多样性。本航次期间，研究人员从多个海洋点位采集了环境DNA样本，随后采用靶向鱼类线粒体12S rRNA基因特定区域的MiFish引物开展宏条形码（metabarcoding）分析。所获得的环境DNA序列在Illumina MiSeq测序平台上完成测序，生成了高质量的fastq文件。本数据集提供了包含测序读长、质量评分在内的关键元数据，这些数据对于研究鱼类多样性的时空分布模式至关重要。这套完整的环境DNA数据集可作为生态学与保护研究的宝贵资源，能够以高分辨率和高精度实现鱼类物种鉴定及海洋生态系统监测。