RNA-seq study on the placentomes collected from the cows with or without retained placenta. RNA-seq study on the placentomes collected from the cows with or without retained placenta

We conducted an RNA-sequencing on the placentomes that were collected from 4 and 3 cows with and without retained placenta, respectively. Placentomes were collected from the cows within 30 minutes after parturition and samples were taken from the feto-maternal junction sites of the placentomes. These samples were used for RNA-sequencing and the results showed 240 genes that were differentially expressed between the two groups. These genes could be candidate genes that are involved in the development of placental retention in cattle. Overall design: Identifying the genes that are differentially expressed in the placentome of cows with or without retained placenta.

本研究对分别来自4头患胎衣不下（retained placenta）的奶牛与3头健康对照奶牛的胎盘叶（placentome）开展了RNA测序（RNA-sequencing）。所有胎盘叶样本均采集于奶牛分娩后30分钟内，且取自胎盘叶的胎母连接位点。上述样本经RNA测序分析后，结果显示两组间共存在240个差异表达基因（differentially expressed genes）。这些基因可作为参与奶牛胎衣不下发病进程的候选基因。整体实验设计：鉴定胎衣不下与健康奶牛胎盘叶中的差异表达基因。