Inflammatory gene expression profiling in peripheral blood from patients with Alzheimer’s disease reveals key pathways and hub genes with potential diagnostic utility. Inflammatory gene expression profiling in peripheral blood from patients with Alzheimer’s disease reveals key pathways and hub genes with potential diagnostic utility

Late-onset Alzheimer disease (LOAD) is the most common neurodegenerative disorder worldwide. Recent studies have shown that differential expression in genes (DEG) related to inflammatory response may result associated with disease onset and progression. This study aimed to explore the molecular pathogenesis of LOAD-related inflammation through next-generation sequencing, to assess RNA expression profiles in Alzheimer's disease patients and healthy controls. Overall design: RNA was isolated from whole blood from 5 LOAD cases and 10 controls (all female. Mean age 76.3±3.5). The amplicon sequencing approach using 20812 probes sets specific to human mRNA was applied to assess gene expression profiles, and functional annotation was performed.

晚发性阿尔茨海默病（Late-onset Alzheimer disease, LOAD）是全球范围内最常见的神经退行性疾病。近期研究表明，与炎症反应相关的差异表达基因（differentially expressed genes, DEG）可能与疾病的发生及进展相关。本研究旨在通过下一代测序（next-generation sequencing, NGS）探究LOAD相关炎症的分子发病机制，并分析阿尔茨海默病患者与健康对照者的RNA表达谱。实验整体设计：从5例LOAD患者与10例健康对照者的全血中提取RNA（所有受试者均为女性，平均年龄76.3±3.5岁）。本研究采用针对人类mRNA设计的20812个探针组构建扩增子测序方法，以检测基因表达谱并完成功能注释。