Co-deregulated genes in lung cancer

Here, we reanalysed a total of 27 GEO studies to identify and annotate lung cancer versus normal signatures, as well as single-gene perturbation, and single-drug perturbation signatures. Differential expression within each category was computed using GEO2Enrichr and the Characteristic Direction method. We identified the co-DEGs across different studies within each category, and their upstream regulating kinases and TFs using Expression2Kinases.

本研究重新分析了共计27项基因表达综合数据库（Gene Expression Omnibus，GEO）相关研究，旨在鉴定并注释肺癌与正常组织的差异特征基因集，以及单基因扰动、单药物扰动的特征基因集。研究通过GEO2Enrichr与特征方向法（Characteristic Direction method）计算每一类样本的差异表达水平，并借助Expression2Kinases工具，鉴定出每一类下不同研究间的共差异表达基因（co-DEGs），及其上游调控激酶与转录因子（Transcription Factors，TFs）。