miR-1199-5p and Zeb1: a novel double-negative feedback coordinating EMT and tumour cell invasion (mRNA-seq)

We investigated the effect of miR-1199-5p, miR-200b-3p and miR-429-3p on gene expression profiles during TGFbeta-induced EMT in normal murine mammary gland cells by using the mRNA-sequencing. Our analysis demonstrates that miR-1199-5p and both miR-200 family members share only 6 target genes, indicating that besides regulating Zeb1 expression they exert distinct functions during EMT. Overall design: mRNA profiles of NMuMG cells transiently overexpressing miR-1199-5p, miR-200b-3p or miR-429-3p and treated with TGFbeta for 4 days

本研究通过mRNA测序技术，探究了miR-1199-5p、miR-200b-3p及miR-429-3p对转化生长因子β（transforming growth factor β, TGFβ）诱导的正常小鼠乳腺细胞上皮间质转化（epithelial-mesenchymal transition, EMT）过程中基因表达谱的影响。本研究分析显示，miR-1199-5p与miR-200家族的两个成员仅共享6个靶基因，这表明二者除均可调控ZEB1（锌指E盒结合同源框1, zinc finger E-box-binding homeobox 1）的表达外，在上皮间质转化过程中还发挥着截然不同的功能。实验设计概况：对瞬时过表达miR-1199-5p、miR-200b-3p或miR-429-3p的NMuMG细胞施加转化生长因子β处理4天，采集其mRNA表达谱数据。