Temperature dependence of the transcriptome in cultured Drosophila S2R+ cells

Cells in ectothermic organisms often maintain homeostatic function over a considerable range of ambient temperatures. However, as temperature has pronounced effects on all biological processes, but not necessarily in a uniform manner on each of the myriad of distinct processes, cellular acclimation to ambient temperature change is predicted to involve complex regulation. To assess the effects of temperature change within the readily tolerated temperature range on the transcriptome, we have performed expression profiling with S2R+ cells, which are derived from the ectothermic organism Drosophila melanogaster. Aliquots of S2R+ cells were plated and shifted to different temperatures (11, 14, 25, and 30°C). Twenty-four hours after the temperature shift, total RNA was isolated and processed for hybridization of microarrays. Three replicate experiments were preformed.

变温动物（ectothermic organism）体内的细胞通常可在较宽的环境温度范围内维持稳态功能。然而，温度对所有生物学过程均存在显著影响，但这种影响并非以统一模式作用于每一类各异的生物学过程，因此推测细胞对环境温度变化的适应过程涉及复杂的调控机制。为探究易耐受温度范围内的温度变化对转录组（transcriptome）的影响，我们以源自黑腹果蝇（Drosophila melanogaster）的S2R+细胞为材料开展了表达谱分析。将S2R+细胞等分后铺板培养，随后转移至不同温度（11、14、25及30℃）环境中。温度转移24小时后，提取总RNA并制备用于基因芯片（microarray）杂交的样品。共完成3次重复实验。