Induction of E. coli BL21(DE3) with various IPTG concentrations and incubation temperatures

Cultivation of Escherichia coli BL21(DE3) expressing L-Asparginase type 2 from E. coli inserted in a plasmid pET28a. Cultivation at 23°C (Experiment group R2006), 30°C (Experiment group R2005), or 37°C (Experiment group R2004). Induction with IPTG at initial time at concentration: 0, 20, 60, 100, or 1000 µmol/L.

本数据集针对携带插入至pET28a质粒（plasmid pET28a）的大肠杆菌来源L-天冬酰胺酶2型（L-Asparginase type 2）编码基因的大肠杆菌BL21(DE3)（Escherichia coli BL21(DE3)）菌株开展培养实验。实验设置三组培养温度：23℃（实验组R2006）、30℃（实验组R2005）与37℃（实验组R2004）；于培养初始时刻采用异丙基-β-D-硫代半乳糖苷（IPTG）进行诱导，诱导浓度为0、20、60、100及1000 µmol/L。