Endogenous retroviruses synthesize heterologous chimeric RNAs to reinforce human early embryo development (HNRNPU KD in 8CLC)

RNA pulldown-MS showed that HNRNPU was the most enriched RNA binding protein to complex with MLT2A1 RNA. Thus, to study the function of HNRNPU, RNA-seq was performed for siNC and siHNRNPU samples in 8C-like cells Overall design: Differentially expressed genes and TEs were calculated by comparing the siHNRNPU with siNC samples

RNA下拉质谱（RNA pulldown-MS）实验结果表明，异质核核糖核蛋白U（HNRNPU）是与MLT2A1 RNA形成复合物的富集程度最高的RNA结合蛋白。为研究HNRNPU的功能，本研究对8细胞样细胞（8C-like cells）中的阴性对照小干扰RNA（siNC）组与靶向HNRNPU的小干扰RNA（siHNRNPU）组样本开展了RNA测序（RNA-seq）。实验整体设计：通过对比siHNRNPU组与siNC组样本，计算得到差异表达基因与转座因子（transposable elements, TEs）的表达差异。