Raw Data from: Targeted Screening Studies on Endemic Propolis Extracts for Selective Cytotoxicity Against Melanoma Cells

The aim of this study was to determine the cytotoxicity of propolis from endemic areas of Nepal, Greece, Kazakhstan, Ukraine, and Georgia against the malignant melanoma cell lines: A375 and SK-MEL-3, as well as against normal human skin fibroblasts (BJ). Within this study following propolis extracts were tested: Nepal – NE-TRI (no. 1), Greece – GR-PA (no. 8), Kazakhstan – region Bozovoe – KA-BO (no. 7), Ukraine – UK-TA (no. 9), Georgia – region Orgora – GE-OR (no. 4), Georgia region Ota – GE-OT (no. 3), Georgia – region Qvakhvreli sample 1 – GE-Q1 (no. 2), Georgia – region Qvakhvreli sample 2 – GE-Q2 (no. 6), Georgia – region Vazisubani sample 1 – GE-VA1 (no. 5), Georgia – region Vazisubani sample 2 – GE-VA2 (no. 10). Biological properties were evaluated by analyzing cell viability, apoptosis (fluorescent staining and confocal laser scanning microscope imaging), and caspase-6 activity (fluorometric assay). The results showed that 3 propolis samples from Georgian endemic regions (GE-OR, GE-VA1, and GE-Q2) exhibited the highest anticancer activity, with low toxicity towards normal cells (Si >1).

Folder entitled ‘Cytotoxicity evaluation’ contains 3 folders for 3 cell lines used: BJ, A375, and SK-MEL-3. Each folder (named with the cell line code) contains several Microsoft Excel files (.xlsx) presenting raw MTT results for various propolis extracts (the name of the file indicates cell line used and propolis tested: NE-TRI (no. 1), GR-PA (no. 8), KA-BO (no. 7), UK-TA (no. 9), GE-OR (no. 4), GE-OT (no. 3), GE-Q1 (no. 2), GE-Q2 (no. 6), GE-VA1 (no. 5), GE-VA2 (no. 10)).

Folder entitled ‘Apoptosis Necrosis Assay’ contains raw confocal laser scanning microscope images (.TIF) of the propolis-treated cells. The name of the image indicates cell line used (BJ, A375, and SK-MEL-3) and propolis tested (no. 4 – GE-OR, no. 5 – GE-VA1, and no. 6 – GE-Q2).

Microsoft Excel file (.xlsx) entitled ‘Caspase 6 48h’ contains the raw results of Caspase-6 activity assay for selected propolis extracts (no. 4 – GE-OR, no. 5 – GE-VA1, and no. 6 – GE-Q2).

本研究旨在测定采自尼泊尔、希腊、哈萨克斯坦、乌克兰及格鲁吉亚特有产区的蜂胶，针对恶性黑色素瘤细胞系A375、SK-MEL-3，以及正常人皮肤成纤维细胞(BJ)的细胞毒性。本研究共测试以下蜂胶提取物：尼泊尔来源 NE-TRI（编号1）、希腊来源 GR-PA（编号8）、哈萨克斯坦博佐沃耶产区 KA-BO（编号7）、乌克兰来源 UK-TA（编号9）、格鲁吉亚奥尔戈拉产区 GE-OR（编号4）、格鲁吉亚奥塔产区 GE-OT（编号3）、格鲁吉亚克瓦赫夫雷利样本1 GE-Q1（编号2）、格鲁吉亚克瓦赫夫雷利样本2 GE-Q2（编号6）、格鲁吉亚瓦济苏巴尼样本1 GE-VA1（编号5）、格鲁吉亚瓦济苏巴尼样本2 GE-VA2（编号10）。

本研究通过以下方式评估生物学特性：细胞活力分析、细胞凋亡检测（荧光染色结合共聚焦激光扫描显微镜成像）以及半胱氨酸天冬氨酸蛋白酶-6（caspase-6）活性检测（荧光分析法）。结果显示，来自格鲁吉亚特有产区的3份蜂胶样品（GE-OR、GE-VA1及GE-Q2）展现出最高的抗癌活性，且对正常细胞毒性较低（Si>1）。

名为"细胞毒性评价"的文件夹包含3个子文件夹，分别对应本次实验所用的3种细胞系：BJ、A375及SK-MEL-3。每个以细胞系代码命名的子文件夹内均存储有若干Microsoft Excel（.xlsx）文件，记录了不同蜂胶提取物的MTT原始检测结果，文件名可反映所用细胞系及测试蜂胶：NE-TRI（编号1）、GR-PA（编号8）、KA-BO（编号7）、UK-TA（编号9）、GE-OR（编号4）、GE-OT（编号3）、GE-Q1（编号2）、GE-Q2（编号6）、GE-VA1（编号5）、GE-VA2（编号10）。

名为"凋亡与坏死检测"的文件夹包含经蜂胶处理的细胞的共聚焦激光扫描显微镜原始图像（.TIF），图像文件名可反映所用细胞系（BJ、A375、SK-MEL-3）及测试蜂胶（编号4——GE-OR、编号5——GE-VA1、编号6——GE-Q2）。

名为"半胱氨酸天冬氨酸蛋白酶6 48h"的Microsoft Excel（.xlsx）文件，收录了所选蜂胶提取物（编号4——GE-OR、编号5——GE-VA1、编号6——GE-Q2）的caspase-6活性检测原始结果。