COMPOSITIONS AND METHODS FOR GENOME EDITING

The present disclosure provides compositions， methods， and systems for targeted plant genome editing. In some embodiments， the present disclosure provides recombinant self-replicating RNAs derived from a plant virus vector， such as those derived from Tobacco Mosaic Virus (TMV). In some embodiments， the recombinant self-replicating RNAs direct the expression of both a CRISPR endonuclease and a guide RNA capable of directing sequence-specific binding of the CRISPR endonuclease to a target DNA in the genome of plant cell. The present disclosure provides compositions， methods， and systems for targeted plant genome editing. In some embodiments， the present disclosure provides recombinant self-replicating RNAs derived from a plant virus vector， such as those derived from Tobacco Mosaic Virus (TMV). In some embodiments， the recombinant self-replicating RNAs direct the expression of both a CRISPR endonuclease and a guide RNA capable of directing sequence-specific binding of the CRISPR endonuclease to a target DNA in the genome of plant cell.

本公开提供了用于靶向植物基因组编辑的组合物、方法与系统。在部分实施例中，本公开提供了源自植物病毒载体的重组自我复制RNA，例如源自烟草花叶病毒（Tobacco Mosaic Virus, TMV）的重组自我复制RNA。在部分实施例中，该重组自我复制RNA可同时引导CRISPR核酸内切酶与向导RNA（guide RNA）的表达，其中向导RNA可引导CRISPR核酸内切酶与植物细胞基因组内的靶DNA发生序列特异性结合。本公开提供了用于靶向植物基因组编辑的组合物、方法与系统。在部分实施例中，本公开提供了源自植物病毒载体的重组自我复制RNA，例如源自烟草花叶病毒（Tobacco Mosaic Virus, TMV）的重组自我复制RNA。在部分实施例中，该重组自我复制RNA可同时引导CRISPR核酸内切酶与向导RNA（guide RNA）的表达，其中向导RNA可引导CRISPR核酸内切酶与植物细胞基因组内的靶DNA发生序列特异性结合。