Expression analysis of WT and Zbtb4 -/- mouse primary fibroblasts

ZBTB4 is a mammalian transcription factor with Zinc fingers and a BTB/POZ domain, which can bind methylated CpGs, as well as certain unmethylated consensus sequences. ZBTB4 is frequently downregulated in human cancers, but it is unclear whether this is a cause or consequence of transformation. To investigate the role of ZBTB4 in normal and pathological conditions, we generated Zbtb4-/- mice To address the Zbtb4 dependant transcriptome, a 10 chip study has been realized using total RNA recovered from wild type and Zbtb4 knock-out mouse embryonic fibroblasts (MEFs)

ZBTB4是一类含有锌指结构域与BTB/POZ结构域的哺乳动物转录因子，能够结合甲基化CpG位点以及特定的未甲基化共有序列。ZBTB4在人类恶性肿瘤中常呈表达下调状态，但目前尚不明确该现象是肿瘤转化的诱因还是结果。为探究ZBTB4在生理与病理状态下的生物学功能，本研究构建了Zbtb4基因敲除（Zbtb4-/-）小鼠。为解析Zbtb4依赖的转录组，本研究利用从野生型与Zbtb4基因敲除小鼠胚胎成纤维细胞（mouse embryonic fibroblasts, MEFs）中提取的总RNA，开展了10芯片研究。