Expression profiling of IL6 and Myc TG mouse plasmacytoma

To identify differentially expressed genes in mouse IL6 and Myc double TG plasmacytoma, total RNAs from mouse plasmacytoma generated from IL6 + Myc TG mice were applied to NIAID mouse gene expression arrays. The transcriptome in IL6+ Myc TG mouse PCT was compared to transcriptome in IL6 TG mouse PCT Eleven set of IL6+Myc TG PCTs and ten set of IL6 TG PCTs were used. Total RNAs from each mouse labelled with Cy3, and reference RNAs labeled with Cy5 were hybridzed to spotted oligomicroarray.

为鉴定小鼠IL6与Myc双转基因浆细胞瘤中的差异表达基因（differentially expressed genes），本研究将IL6+Myc TG（transgenic）小鼠诱导产生的浆细胞瘤总RNA应用于美国国家过敏和传染病研究所（National Institute of Allergy and Infectious Diseases, NIAID）小鼠基因表达芯片。将IL6+Myc TG小鼠浆细胞瘤的转录组（transcriptome）与IL6 TG小鼠浆细胞瘤的转录组进行比对，本次实验共使用11组IL6+Myc TG浆细胞瘤样本与10组IL6 TG浆细胞瘤样本。将每只小鼠的总RNA以Cy3标记，参考RNA以Cy5标记后，与点样寡核苷酸微阵列（spotted oligomicroarray）进行杂交。