Detection of CYP2C19*2 allele among Helicopacter pylori-infected patients in two tertiary hospitals in Khartoum-Sudan

Antral biopsies were collected from 30 patients attending endoscopy units. EXtraction of DNA was performed through QIAampR DNA mini kit. Samples were screened for Urease C (UreC) gene of H.pylori using conventional PCR, Detection of CYP2C19*allele was performed in positive H.pylori samples using Real time-PCR. Ethical clearance was obtained from the scientific committee of the Virology department of central laboratories- Ministry of higher education and Research. Consent was also taken from hospital managers and patients after explanation of research purpose and procedures.

本研究从30名接受内镜检查的患者处采集胃窦活检组织。采用QIAamp® DNA迷你试剂盒（QIAampR DNA mini kit）完成基因组DNA提取。通过常规聚合酶链式反应（PCR）对样本进行幽门螺杆菌（Helicobacter pylori，H. pylori）的尿素酶C（Urease C，简称UreC）基因筛查；针对幽门螺杆菌检测呈阳性的样本，采用实时PCR（Real time-PCR）开展CYP2C19*等位基因的检测。本研究已获得高等教育与研究部中央实验室病毒学系科学委员会的伦理审查批准。在向医院管理人员及患者详细阐明研究目的与实验流程后，均已获得其知情同意。