Data Sheet 1_Personalized audiovisual gamma stimulation enhances neural connectivity and entrainment beyond fixed 40 Hz protocols.docx

BackgroundConventional 40 Hz gamma stimulation is applied across individuals, potentially overlooking inter-individual neural variability.
ObjectiveThis study evaluated conversation gamma frequency (CGF)–a personalized gamma frequency derived from task engagement–against the fixed 40 Hz and individual gamma frequency (IGF) derived from auditory responses.
MethodsIn Experiment 1, gamma center frequencies were measured under resting, reading, and conversation conditions. In Experiment 2, EEG was used to compare neural entrainment effects across CGF, 40 Hz, and IGF conditions.
ResultsConversation gamma frequency stimulation induced stronger neural activation and functional connectivity in the frontal, temporal, and parietal cortices compared to 40 Hz or IGF. Theta-gamma coupling analysis revealed significantly increased phase synchronization under CGF compared to 40 Hz with enhanced connectivity. However, entrainment declined as the frequency difference between CGF, and 40 Hz increased, emphasizing the limitation of fixed-frequency stimulation.
ConclusionThese findings provide EEG-based mechanistic evidence that individualized gamma stimulation may represent a hypothesis-generating strategy for future neurorehabilitation research in aging and neurodegenerative conditions.

研究背景：传统40Hzγ（gamma）频率刺激广泛应用于各类受试者，但该方案可能忽略了个体间的神经异质性。
研究目的：本研究对任务态下生成的个性化γ频率——会话γ频率（conversation gamma frequency，CGF），与固定40Hzγ频率以及基于听觉反应的个体γ频率（individual gamma frequency，IGF）展开对比评估。
研究方法：实验1中，研究人员在静息、阅读及会话三种状态下测定了γ中心频率。实验2中，通过脑电图（electroencephalogram，EEG）比较了CGF、40Hz与IGF三种刺激条件下的神经夹带效应。
研究结果：相较于40Hz或IGF刺激，会话γ频率刺激可在额叶、颞叶及顶叶皮层诱发更强的神经激活与功能连接。θ-γ耦合分析显示，与40Hz刺激相比，CGF刺激下的相位同步性显著提升，且连接性增强。但随着CGF与40Hz的频率差增大，神经夹带效应会出现衰减，这凸显了固定频率刺激的局限性。
研究结论：本研究结果提供了基于脑电图的神经机制证据，证实个性化γ频率刺激或可作为未来衰老与神经退行性疾病神经康复研究的假说生成策略。