RNA Sequencing analysis of different genetically characterized lung cancer cell lines. RNA Sequencing analysis of different genetically characterized lung cancer cell lines

We performed RNA sequencing to assess changes in gene expression in lung cancer cell lines with MET genetic alterations with or without co-occurrence of JAK2 inactivating mutations. Different treatments have been administrated to activate or inhibit selected pathways in order to define MET signature and IFNg (or JAK/STAT) signature. Overall design: Differential expression analysis of RNA sequencing of 4 different lung cancer cell lines with MET genetic alterations treated with different treatements to activate or inhibit selected pathways

本研究通过RNA测序（RNA sequencing），评估携带MET基因（MET）遗传变异、伴或不伴JAK2（JAK2）失活突变的肺癌细胞系的基因表达变化。为明确MET基因特征与干扰素γ（IFNγ，或JAK/STAT通路）特征，本研究采用不同处理手段激活或抑制选定通路。整体实验设计：对4株携带MET基因遗传变异的肺癌细胞系进行RNA测序，分析其经不同处理以激活或抑制特定通路后的差异表达情况。