Additional file 1 of Chalkiness and premature controlled by energy homeostasis in OsNAC02 Ko-mutant during vegetative endosperm development

Additional file 1: Figure S1. The mutant tags of target sites and mutants detection by PCR sequencing. Figure S2. The yield characters of T2 N2 /N3 mut (95 DAG) in field (95 DAG in 2021, 125 DAG in 2020). Figure S3. The homologous clusters of OsNAC family genes by phylogenetic analysis. Figure S4. The homologous analysis of NAC in plant. The Gene ID are according to the database RAP (rapdb.dna.affrc.go.jp), and (OsNAC02, Os02g0594800), (OsNAC06, Os06g0267500) in NCBI of homologous analysis. Figure S5. The ABRE/ G-box motif locations on the promoter regions of OsNAC02 regulated genes. Figure S6. The chalky phenotype of N2/N3 mut seeds (T2) in 2021. Figure S7. The histological analysis during endosperm development. Figure S8. The PCA clusters based on RNA-seq analysis. Figure S9. The heatmap diagram of DEGs in N2/N3 versus WT. Figure S10. The genes of 39 ECs in Sucrose and Starch metabolic pathway in Mapman of N2 vs WT seeds (3DAP) are verified by qRT-PCR. Figure S11. The antagonism between energy storage and development requirement under stress in Ko-Osnac02 mutant.

附加文件1：图S1 靶位点突变标签及通过聚合酶链式反应（Polymerase Chain Reaction，PCR）测序开展的突变体鉴定。图S2 田间种植的T2代N2/N3突变体的产量性状（2020年为发芽后125天，2021年为发芽后95天（DAG））。图S3 基于系统发育分析的OsNAC家族基因同源聚类。图S4 植物NAC基因同源性分析。基因ID参照RAP数据库（rapdb.dna.affrc.go.jp），同源性分析涉及的基因包括NCBI数据库中收录的(OsNAC02, Os02g0594800)与(OsNAC06, Os06g0267500)。图S5 OsNAC02调控基因启动子区域的ABRE/G-box基序定位。图S6 2021年T2代N2/N3突变体种子的垩白表型。图S7 胚乳发育过程的组织学分析。图S8 基于RNA测序（RNA-seq）分析的主成分分析（Principal Component Analysis，PCA）聚类结果。图S9 N2/N3与野生型（Wild Type，WT）间差异表达基因（Differentially Expressed Genes，DEGs）的热图。图S10 采用实时定量逆转录聚合酶链式反应（quantitative Real-Time Polymerase Chain Reaction，qRT-PCR）验证N2与WT授粉后3天（3DAP）种子蔗糖与淀粉代谢通路中Mapman注释的39个ECs基因。图S11 OsNAC02基因敲除突变体（Ko-Osnac02）中能量储存与胁迫条件下发育需求间的拮抗作用。