Time resolved DIA MS analysis of FZO1 depletion in s. cerevisiae

Mitochondrial biogenesis and inheritance must be carefully regulated alongside cell division to ensure proper mitochondrial function and cell survival. The dynamics of the mitochondrial network, including fusion and fission, play a crucial role in mitochondrial inheritance by facilitating the distribution and quality control of mitochondria. Here, we investigate the consequences of impaired mitochondrial fusion through depletion of Fzo1, which is essential for mitochondrial outer membrane fusion in s. cerevisiae. We use the AID (auxin-induced degron) system, which enables induced protein degradation and monitor changes in the proteome up to 21 hours after depletion.

线粒体生物发生与遗传必须与细胞分裂协同进行精密调控，以保障线粒体的正常功能及细胞存活。线粒体网络的动态变化（包括融合与分裂）通过促进线粒体的分布与质量管控，在线粒体遗传过程中发挥关键作用。本研究通过降解酿酒酵母（S. cerevisiae）中线粒体外膜融合的必需蛋白Fzo1，探究线粒体融合受损所带来的效应。我们采用可诱导蛋白质降解的生长素诱导降解子系统（auxin-induced degron，AID），对Fzo1降解后长达21小时内的蛋白质组变化进行监测。