Single-cell RNA-seq of the testis sample of ICR mice treated with busulfan and/or alginate oligosaccharides. Single-cell RNA-seq of the testis sample of ICR mice treated with busulfan and/or alginate oligosaccharides

We profiled a single-cell RNA-seq on ICR mouse testis treated with busulfan and or Alginate Oligosaccharides (AOS) from 3 weeks of age to 8 weeks of age (for 5 weeks) to study the rescue effect of AOS on the spermatogenesis disrupted by busulfan. We collected single cell from testis from the four groups AOS 0 (control), AOS 10 (AOS 10 mg/kg body weight (BW)), B+A 0 (busulfan alone), B+A 10 (busulfan plus AOS 10 mg/kg BW). The the cells were further captured with 10x Genomics based single-cell system. By using single-cell RNA seq, we found busulfan disrupted spermatogenesis by the dramatical decrease in germ cells. AOS significantly increased the number of germ cells and also promoted the germ cell development. Besides, we provide candidate master regulons regulated by AOS. Our data here offers the opportunity for treating of azoospermia or oligospermia. Overall design: We totally analyzed ~27,000 single cell transcriptome profile from ICR mouse testis from the four treatment group.

本研究针对自3周龄至8周龄（共计5周）接受不同处理的ICR小鼠睾丸组织开展单细胞RNA测序（single-cell RNA-seq），处理方式涵盖空白对照、单用藻寡糖（Alginate Oligosaccharides, AOS）、单用白消安（busulfan）以及白消安联合AOS，旨在探究AOS对白消安诱导的生精功能障碍的挽救作用。我们从四组实验对象中收集睾丸单细胞样本，分别为：AOS 0组（空白对照组）、AOS 10组（以10 mg/kg体质量（body weight, BW）剂量的AOS处理组）、B+A 0组（仅白消安处理组）、B+A 10组（白消安联合10 mg/kg体质量AOS处理组）。后续通过基于10x Genomics的单细胞捕获系统完成细胞分选与捕获。通过单细胞RNA测序分析，我们发现白消安可通过显著降低生殖细胞数量破坏生精过程；而AOS可显著提升生殖细胞数量，并促进生殖细胞发育。此外，本研究还鉴定出受AOS调控的候选核心调控元（master regulons）。本数据集可为无精子症（azoospermia）或少精子症（oligospermia）的临床治疗提供新的研究契机。整体实验设计：本研究共分析了来自四组处理的ICR小鼠睾丸的约27000个单细胞转录组图谱。