Influence of Erythropoietin on Microvesicles Derived from Mesenchymal Ste cells(MSC) protecting renal function of Chronic Kidney Disease

miRNA profiles of the MSC-MVs and EPO-MVs were analyzed with a quantitative PCR (qPCR)-based array of the whole mice genome. Further analysis revealed differences in the miRNAs of 212 EPO-MVs (fold change ≥ 1.5 compared to the MSC-MVs), which constituted approximately 22.64% of all of the evaluated mouse miRNAs. Of all of the differences, 70.28% of the changes in the EPO-MV group involved upregulation To study the differential miRNA expression in MV and EPO-MV which might contributed to the better treatment in chronic kidney disease, we performed miRNA expression profiling of the culture supernatant of MSC with or without EPO incubation using the miRCURY LNA Array (v.18.0) (Exiqon, Vedbaek, Denmark).

本研究采用基于实时定量聚合酶链式反应（quantitative PCR, qPCR）的小鼠全基因组芯片，对间充质干细胞来源微囊泡（MSC-MVs）与促红细胞生成素（erythropoietin, EPO）处理的间充质干细胞微囊泡（EPO-MVs）的微小RNA（microRNA, miRNA）表达谱进行检测。进一步分析显示，相较于MSC-MVs，EPO-MVs中存在212个差异表达的miRNA（倍数变化≥1.5），约占所有检测小鼠miRNA的22.64%。在所有差异表达的miRNA中，EPO-MV组中有70.28%的表达变化表现为上调。为探究微囊泡（MVs）与EPO-MVs中差异表达的miRNA是否可改善慢性肾脏疾病的治疗效果，本研究使用miRCURY锁核酸（LNA）芯片（版本18.0，购自丹麦维兹拜克市艾克信公司（Exiqon）），对经EPO孵育与未孵育的间充质干细胞的培养上清液开展miRNA表达谱分析。