Adverse effect of folic acid supplementation on cilia

We report that fortified levels of folic acid adversely affect cilia strucure and function. This data set agrees with previous experiements which have demonstrated that elevated folic acid levels can increase transcription variability on a genome-wide level. Furthermore, we demonstrate that among these dysregulated genes, genes contained within SYSgold cilia database are proportionally over-represented. This over-representation of cilia genes among dysregulated genes may play a key role in ciliopathys' sensitivity to elevated folic acid levels. Examination of mice fed two levels of folic acid (moderate[8 samples] and enriched[8samples]) that are either WT(4 samples per diet) or homozygous IFT88-null (4 samples per diet)

本研究证实，强化剂量的叶酸可对纤毛结构与功能产生负面影响。本数据集与既往相关实验结果相符：已有研究表明，升高的叶酸水平可在全基因组范围内增加转录变异程度。此外，本研究发现，在上述表达失调的基因中，SYSgold纤毛数据库收录的纤毛相关基因占比显著偏高。此类表达失调基因中纤毛基因的富集现象，可能是纤毛病对升高的叶酸水平具有易感倾向的关键原因。本研究对饲喂两种叶酸水平日粮的小鼠进行了分组检测：两种日粮分别为中等剂量组（8份样本）与高剂量强化组（8份样本），每组日粮下的小鼠又分为野生型（Wild Type，WT，每组4份样本）与IFT88基因敲除纯合子（IFT88-null，每组4份样本）两个亚组。