Pro-inflammatory stimulation of human skin-derived precursor cells modulates several of their immunology-related pathways.

Human skin-derived precursor cells (hSKP) are a stem cell population that represents key candidates for cell based-therapy. Inflammation, however, is often present in situations where cellular replacement therapy is required. These inflammatory conditions, and more specifically the presence of the cytokine interferon (IFN)-γ, might result in an increase of MHC class II antigens in hSKP-derived grafts and facilitate their rejection. We used microarray analyses to confirm the activated status of the pro-inflammatory condition, induced by exposure to a cocktail of pro-inflammatory cytokines (IL-1β, IFN-γ, TNFα and IFN-α). Consequently, we investigated the modulation by inflammation of canonical pathways related to immunology in hSKP. hSKP obtained from 3 different donors (passage 4) were cultivated in the presence or absence of the pro-inflammatory cocktail. Control and pro-inflammatory stimulated samples were collected 18h after treatment.

人皮肤来源前体细胞（human skin-derived precursor cells，hSKP）是一类干细胞群，亦是细胞疗法的关键候选细胞。然而，在需要开展细胞替代治疗的场景中，炎症反应往往存在。这类炎症状态，尤其是细胞因子干扰素γ（interferon-γ，IFN-γ）的存在，可能会导致hSKP来源移植物中主要组织相容性复合体II类分子（major histocompatibility complex class II，MHC II）的表达上调，并促进移植物的免疫排斥。本研究采用基因芯片（microarray）分析，验证了促炎细胞因子组合（IL-1β、IFN-γ、TNFα及IFN-α）刺激所诱导的促炎状态的激活情况。基于此，本研究探究了炎症反应对hSKP中免疫学相关经典通路的调控作用。本研究从3名不同供者处获取第4代hSKP，分别在含或不含该促炎细胞因子组合的培养基中培养，并于处理后18小时收集对照组与促炎因子刺激组的样本。