PDX1-/NKX6.1+ progenitors derived from human pluripotent stem cells as a novel source of insulin-secreting cells

PDX1-/NKX6.1+ progenitors derived from hESCs were differentiated to pancreatic beta cell stage where they generated glucose-responsive, insulin-secreting cells. The transcriptome profile of hESC- derived PDX1-/NKX6.1+ progenitors were compared with hPSC-derived PDX1+/NKX6.1+ pancreatic progenitors as described in the article entitled "<strong>PDX1^-/NKX6.1⁺progenitors derived from human pluripotent stem cells as a novel source of insulin-secreting cells</strong>" by Memon et al. 2020. Furthermore, beta cells derived from PDX1-/NKX6.1+ progenitors (3D-beta) were compared with their own endocrine progenitors at an earlier stage (stage 5, 3D-S5) to map their development. The file name is : Sample name_replicate_sample ID_read direction_001 where: -3D-beta: insulin-expressing cells derived from self-detached, 3D, PDX1-/NKX6.1+ progenitors -3D-S5: endocrine progenitors from stage 5, derived from self-detached, 3D, PDX1-/NKX6.1+ progenitors - RNA-seq data were generated from two biological replicates in most cases. - Read direction: R1 (Forward), R2 (Reverse).