Changes in circulating miRNA expression in right heart remodeling due to pulmonary hypertension. Changes in circulating miRNA expression in right heart remodeling due to pulmonary hypertension

Clinical practice currently faces a significant shortfall in specific biomarkers needed for diagnosing right ventricular (RV) remodeling in patients with pulmonary hypertension (PH). MiRNAs are crucial regulators of cardiovascular development, influencing processes like angiogenesis, cardiomyocyte differentiation, cell cycle, cardiac electrical activity, and cardiomyocyte growth. Furthermore, circulating miRNAs are cell-or tissue-specific, relatively stable in serum, and resistant to degradation factors. These properties make serum miRNAs possible promising biomarkers for predictive diagnosis of cardiovascular disease (CVD). This study systematically screened and identified candidate serum miRNAs as potential diagnostic biomarkers for RV remodeling in PH patients. Overall design: Serum samples from Sprague - Dawley rats surviving 4 weeks after Pulmonary artery banding (PAB) and sham surgery were subjected to miRNA high-throughput sequencing and analyzed for differences.

目前临床实践在诊断肺动脉高压（pulmonary hypertension, PH）患者的右心室重构（right ventricular remodeling, RV）所需的特异性生物标志物方面存在显著缺口。微核糖核酸（miRNAs）是心血管发育的关键调控因子，可调控血管生成（angiogenesis）、心肌细胞分化（cardiomyocyte differentiation）、细胞周期（cell cycle）、心脏电活动（cardiac electrical activity）及心肌细胞生长等生理过程。此外，循环miRNAs（circulating miRNAs）具有细胞或组织特异性，在血清（serum）中相对稳定且可抵御降解因子（degradation factors）的作用，上述特性使血清miRNAs成为心血管疾病（cardiovascular disease, CVD）预测诊断的极具潜力的生物标志物。本研究系统筛选并鉴定了候选血清miRNAs，作为肺动脉高压患者右心室重构的潜在诊断生物标志物。整体实验设计：对接受肺动脉环扎术（pulmonary artery banding, PAB）与假手术（sham surgery）、且术后存活4周的斯普拉格-道利大鼠（Sprague-Dawley rats）的血清样本进行miRNA高通量测序（miRNA high-throughput sequencing），并开展差异表达分析。