Whole ganglia RNA sequencing of C57 BL/6J mice

The study goal is to identify the gene expression profile of iBAT-related ganglia (SG/T1 & T3) and iWAT-related ganglia (T13/L1 & L2). RNA sequencing demonstrated clear separation of gene expression between brown adipose tissue related ganglia (SG and T3) and white adipose tissue related ganglia (T13/L1 and L2). Each sample type also showed clear separation from each other. No significant separation was observed in different genders. All mice were fed with chow (LabDiet Rodent 5001) at room temperature. Ganglia SG, T3, T13/L1, L2 and ganglia fat were isolated from 4 male and 4 female C57 BL/6J mice, followed by RNA extraction. 40 samples were analyzed. There are 8 SG, 8 T3, 8 T13/L1, 8 L2, and 8 ganglia fat samples from 4 males and 4 females.

本研究旨在鉴定诱导性棕色脂肪组织（inducible brown adipose tissue, iBAT）相关神经节（SG/T1与T3）以及诱导性白色脂肪组织（inducible white adipose tissue, iWAT）相关神经节（T13/L1与L2）的基因表达谱。RNA测序结果显示，棕色脂肪组织（brown adipose tissue, BAT）相关神经节（SG与T3）与白色脂肪组织（white adipose tissue, WAT）相关神经节（T13/L1及L2）的基因表达存在清晰的分离现象，且各样本类型之间同样呈现显著的区分。不同性别间未观察到显著的表达分离。所有小鼠均在室温条件下以普通饲料（LabDiet啮齿类饲料5001）进行饲养。研究人员从4只雄性与4只雌性C57 BL/6J小鼠体内分离得到SG、T3、T13/L1、L2神经节及脂肪神经节样本，随后进行RNA提取，共完成40个样本的分析：其中SG、T3、T13/L1、L2及脂肪神经节样本各8个，均来自4只雄性与4只雌性供体小鼠。