Epigenetics CRISPRi screen in mouse neuronal progenitor cells (NPCs)

This dataset examined the epigenetic gene effect on mouse NPCs with Upf2 gene knockout (KO). The sgRNA sequencing results form the epigenetics CRISPRi screen sample are reported. CRISPR sgRNA sequencing: dCas9-Krab-expressing NPCs were harvested from the Upf2(f/f) mice, and transduced with a lentiviral CRISPRi library targeting mouse epigenetics genes (Total 3630 sgRNAs). The integrated sgRNA were amplified from each sample using DCF01 5’-CTTGTGGAAAGGACGAAACACCG-3’ and DCR03 5’- CCTAGGAACAGCGGTTTAAAAAAGC-3’ for high-throughput sequencing (SE75, NextSeq550).

本数据集针对Upf2基因敲除（KO）的小鼠神经前体细胞（Neural Progenitor Cells, NPCs）的表观遗传基因效应展开了研究。本研究报道了来自表观遗传CRISPR干扰（CRISPR interference, CRISPRi）筛选样本的单引导RNA（single guide RNA, sgRNA）测序结果。CRISPR sgRNA测序的具体实验流程如下：从Upf2(f/f)小鼠中分离获得表达dCas9-Krab融合蛋白的神经前体细胞，通过靶向小鼠表观遗传基因的慢病毒CRISPR干扰文库进行转导，该文库共包含3630条sgRNA。从每个样本中扩增整合的sgRNA时，采用引物DCF01（5’-CTTGTGGAAAGGACGAAACACCG-3’）与DCR03（5’-CCTAGGAACAGCGGTTTAAAAAAGC-3’），随后开展高通量测序，测序模式为SE75，使用的测序平台为NextSeq550。