SWATH proteome for the coral reef fish, Acanthochromis polyacanthus, under ocean acidification and ocean warming

Construction of a comprehensive spectral library for the coral reef fish, Acanthochromis polyacanthus, from both DIA and DDA MS runs. The spectral library was then used to quantify proteomes of individual fish exposed to different environmental conditions including ocean acidification and ocean warming. Proteomes were measured for both liver and brain tissue and differential expression between environmental conditions was analyzed.

构建了针对珊瑚礁鱼类多棘雀鲷（Acanthochromis polyacanthus）的综合光谱库，该光谱库基于数据依赖采集（Data Dependent Acquisition，DDA）与数据非依赖采集（Data Independent Acquisition，DIA）两种质谱（Mass Spectrometry，MS）实验流程获取的数据。随后利用该光谱库对暴露于海洋酸化、海洋变暖等不同环境条件的个体鱼类的蛋白质组进行定量分析。本研究分别检测了肝脏与脑组织的蛋白质组，并对不同环境条件下的差异表达情况进行了分析。