Genome-wide identification of mRNA 5-methylcytosine in mammals [RIP_seq]

We report a robust experimental and computational framework to identify mRNA m5C sites with high specificity. A quantitative atlas of RNA m5C in mammals with five thousand high-confidence exonic m5C sites has been developed based on our framework. RNA BS-seq of mRNA from human cell lines, multiple human and mouse tissues. Replicate of each seq is also included. m5C RIP-seq from human cell lines. RIP_seq

本研究报道了一种兼具高特异性的稳健实验与计算框架，可用于识别信使RNA（mRNA）的m5C修饰位点。基于该框架，我们构建了包含五千个高置信度外显子m5C位点的哺乳动物RNA m5C定量图谱。数据集涵盖人细胞系、多个人类及小鼠组织来源的mRNA亚硫酸氢盐测序（RNA BS-seq）数据，且每个测序实验均设有生物学重复。此外还包含人细胞系来源的m5C RNA免疫沉淀测序（m5C RIP-seq）数据，即RIP-seq。