Enrichment of mesophilic and thermophilic mixed microbial cultures for syngas biomethanation in bubble column bioreactors under continuous operation

The dataset compiled in this spreadsheet contains the experimental data used for the development and analysis presented in the associated research paper, regarding the evaluation of bioreactors performance and activity tests. The corpus includes measurements and observations obtained from two experimental setups aimed at investigating syngas biomethanation and the involved metabolic pathways: the continuous bioreactor experiments and batch activity tests in serum bottles.

- Bioreactor Experimental Data [Inoculum, BCB1 and BCB2]

This section documents the operation of two lab-scale bioreactors for syngas biomethanation under different operational conditions. The dataset includes:

- Inoculum characterization
- Sampling dates and experimental timepoints
- Reactor operating conditions
- Inlet and outlet gas compositions (H₂, CO, CO₂, CH₄, O₂, N₂)
- TOC/TN evolution
- TS/VS evolution
- VFAs concentration measurements
- pH evolution

- Serum Bottle Activity Tests [M. activity tests, T. activity tests and Activity tests summary]

This section contains the results of batch tests conducted in sealed serum bottles to evaluate the metabolic activity of microbial cultures under varying substrates addition.
The dataset includes:

- Assay type (regarding substrates employed)
- Initial and final pH measurements
- Headspace pressure measurements
- Headspace gas measurements (CO, H₂, CO₂, CH₄, O₂, N₂)
- Incubation temperture conditions
- VFAs concentration measurements
- Calculated product yields

The data provided insight into microbial activity under the addition of different substrates and were used to evaluate and identify the metabolic pathways involved in the developed syngas biomethanation process.

本电子表格所汇编的数据集，为相关研究论文中开展的生物反应器性能评估与活性测试相关的开发与分析工作提供了实验数据支撑。该数据集涵盖两类实验装置获取的测量数据与观测结果，用于探究合成气（syngas）生物甲烷化（biomethanation）过程及其涉及的代谢途径（metabolic pathways），分别为连续流生物反应器实验与血清瓶批量活性测试。

一、生物反应器实验数据 [接种物（Inoculum）、BCB1与BCB2]
本章节记录了两套实验室规模生物反应器在不同运行条件下开展合成气生物甲烷化的运行过程，数据集包含以下内容：
- 接种物表征
- 采样日期与实验时间节点
- 反应器运行参数
- 进出口气体组分（氢气（H₂）、一氧化碳（CO）、二氧化碳（CO₂）、甲烷（CH₄）、氧气（O₂）、氮气（N₂））
- 总有机碳/总氮（TOC/TN）变化
- 总固体/挥发性固体（TS/VS）变化
- 挥发性脂肪酸（VFAs）浓度测定结果
- pH值变化

二、血清瓶批量活性测试 [微生物活性测试（M. activity tests）、底物活性测试（T. activity tests）及活性测试汇总]
本章节包含在密封血清瓶中开展的批量实验结果，用于评估不同底物添加条件下微生物培养物的代谢活性。数据集包含以下内容：
- 实验类型（基于所使用的底物）
- 初始与最终pH测量值
- 顶空压力测量值
- 顶空气体组分（CO、H₂、CO₂、CH₄、O₂、N₂）
- 培养温度条件
- 挥发性脂肪酸（VFAs）浓度测定结果
- 计算得到的产物得率

本数据集可为不同底物添加条件下的微生物活性研究提供参考，并用于评估与识别所开发的合成气生物甲烷化过程所涉及的代谢途径。