The Co-regulated Genes by Ectopic Expression of MiR-371-372-373 Cluster in HepG2 Cells

MicroRNAs are important cellular regulators and their dysfunctions are associated with various disease. miR-371/372/373 was found co-regulated in HBV-producing HepG2.2.15 cells when compared to its non-HBV producing maternal HepG2 cells. To obtain a glimpse of the potential influence of the enforced miR-371-372-373 cluster in HepG2 gene expression, a two-color Capitalbio 70-mer oligo microarray platform, which contained 21,329 well-characterized human gene probes, was used to identify the differentially expressed genes between miR-371-372-373-HepG2 and mock-HepG2 in two independent biological replicate. miR-371-372-373-HepG2 vs. mock-HepG2

微小核糖核酸（MicroRNAs）是一类重要的细胞调控因子，其表达异常与多种疾病密切相关。研究发现，相较于其亲本的非乙型肝炎病毒（HBV）表达型HepG2细胞，在表达HBV的HepG2.2.15细胞中，miR-371/372/373呈现共调控表达特征。为探明过表达miR-371-372-373基因簇对HepG2细胞基因表达的潜在影响，本研究采用搭载21329个经过充分验证的人类基因探针的双色Capitalbio 70聚体寡核苷酸微阵列平台，通过两次独立的生物学重复实验，鉴定miR-371-372-373-HepG2细胞与mock-HepG2细胞之间的差异表达基因。对比分组：miR-371-372-373-HepG2 vs. mock-HepG2