Additional file 1: Figure S1. of Evolutionary comparison of prenylation pathway in kinetoplastid Leishmania and its sister Leptomonas

Multiple sequence alignment of FTase (α-subunit) of Leishmania donovani (accession number XP_003862625.1, putative protein) with FTase (α-subunit) of Leishmania major (accession number XP_003722277.1, putative protein), Leishmania infantum (accession number XP_001466722.1, putative protein) and its sister Leptomonas (contig_2654, putative protein) showing conserved regions (red colored) based on percentage identity. Figure S2. Multiple sequence alignment of FTase (β-subunit) of Leishmania donovani (accession number XP_003861732.1) with FTase (β-subunit) of Leishmania major (accession number XP_001684151), Leishmania infantum (accession number XP_001470492) and its sister Leptomonas (contig_1135) showing conserved regions (red colored) based on percentage identity. Figure S3. Multiple sequence alignment of GGTase-II (α-subunit) of Leishmania donovani (accession number XM_001468149) with GGTase-II (α-subunit) of Leishmania major (accession number XM_001685808) and Leishmania infantum (accession number XP_001468186) showing conserved regions (red colored) based on percentage identity. Its sister Leptomonas has no α -subunit of GGTase-II. Figure S4. Multiple sequence alignment of GGTase-II (β-subunit) of Leishmania donovani (accession number XP_003864545) with GGTase-II (β-subunit) of Leishmania major (accession number XP_001686510) and Leishmania infantum (accession number XP_001468743) showing conserved regions (red colored) based on percentage identity. Figure S5. Alignment of various Leishmania donovani Rab protein sequences showing sequence similarities and domain conservation among them colored red based in their percentage similarity. Figure S6. Alignment of various Leptomonas Rab protein sequences showing sequence similarities and domain conservation among them colored red based in their percentage similarity. Figure S7. Phylogenetic relationship among Rab sequences of L. major, L. infantum, L. donovani and Leptomonas which is based on multiple sequence alignment (ClustalW algorithm in MEGALIGN with 1000 bootstraps). Figure S8. Circos plot was generated using Circos version 0.60, which is used to determine the similarity between Rab genes of Leishmania donovani and its sister Leptomonas. Where cyan color for all Rabs protein obtained from Leptomonas genome and green color for all Rabs protein obtained from Leishmania donovani genome. Other colors for each different Rabs. (ZIP 81526 kb)

图S1：杜氏利什曼虫（*Leishmania donovani*）法尼基转移酶（FTase）α亚基（登录号XP_003862625.1，推定蛋白）与硕大利什曼虫（*Leishmania major*）FTase α亚基（登录号XP_003722277.1，推定蛋白）、婴儿利什曼虫（*Leishmania infantum*）FTase α亚基（登录号XP_001466722.1，推定蛋白）及其近缘细滴虫（*Leptomonas*）FTase α亚基（重叠群contig_2654，推定蛋白）的多重序列比对，基于序列一致性百分比标注红色保守区域。
图S2：杜氏利什曼虫FTase β亚基（登录号XP_003861732.1）与硕大利什曼虫FTase β亚基（登录号XP_001684151）、婴儿利什曼虫FTase β亚基（登录号XP_001470492）及其近缘细滴虫FTase β亚基（重叠群contig_1135）的多重序列比对，基于序列一致性百分比标注红色保守区域。
图S3：杜氏利什曼虫Ⅱ型香叶酰香叶酰转移酶（GGTase-II）α亚基（登录号XM_001468149）与硕大利什曼虫GGTase-II α亚基（登录号XM_001685808）、婴儿利什曼虫GGTase-II α亚基（登录号XP_001468186）的多重序列比对，基于序列一致性百分比标注红色保守区域；其近缘细滴虫无GGTase-II α亚基。
图S4：杜氏利什曼虫GGTase-II β亚基（登录号XP_003864545）与硕大利什曼虫GGTase-II β亚基（登录号XP_001686510）、婴儿利什曼虫GGTase-II β亚基（登录号XP_001468743）的多重序列比对，基于序列一致性百分比标注红色保守区域。
图S5：多条杜氏利什曼虫Rab蛋白（Rab protein）序列的比对结果，展示各序列间的相似性及结构域保守性，基于序列相似性百分比以红色标记保守区域。
图S6：多条细滴虫Rab蛋白（Rab protein）序列的比对结果，展示各序列间的相似性及结构域保守性，基于序列相似性百分比以红色标记保守区域。
图S7：基于多重序列比对（采用MEGALIGN软件中的ClustalW算法，设置1000次自展重复检验），构建硕大利什曼虫、婴儿利什曼虫、杜氏利什曼虫及细滴虫的Rab序列系统发育关系。
图S8：使用Circos 0.60版本软件绘制的可视化结果，用于分析杜氏利什曼虫与其近缘细滴虫的Rab基因间相似性：其中青色代表细滴虫基因组来源的所有Rab蛋白，绿色代表杜氏利什曼虫基因组来源的所有Rab蛋白，其余颜色对应不同类型的Rab蛋白。本数据集压缩包大小为81526 kb。