Basal Epidermis from UVB-irradiated Mc1r and Kit mutants

Total RNA was extracted from the basal epidermis derived from UVB-irradiated P2.5 normal (Mc1r+/Mc1r+; Kit+/Kit+), Mc1r-mutant (Mc1re/Mc1re) and Kit-mutant (Kit/W-v/Kit/W-v) mice. Biological replicates (cy5) were labelled and co-hybridized with a reference RNA sample (cy3) to a 35K mouse cDNA microarray. A reference experiement design type is where all samples are compared to a common reference. Keywords: reference_design Computed

提取经紫外线B（UVB）照射的出生后第2.5天（P2.5）的正常（Mc1r+/Mc1r+; Kit+/Kit+）、Mc1r突变型（Mc1re/Mc1re）及Kit突变型（Kit/W-v/Kit/W-v）小鼠基底层表皮组织的总RNA（Total RNA）。将生物学重复样本（标记为cy5）与参考RNA样本（标记为cy3）共同杂交至35K点小鼠cDNA微阵列。该实验设计类型为所有样本均与同一公共参考样本进行比对的参考设计方案。关键词：reference_design（参考设计）、Computed（已计算）