Common and Specific Gene Regulatory Programs in Zebrafish Caudal Fin Regeneration at Single-Cell Resolution

Our goal is to delineate the transcriptomic and chromatin accessibility landscape across different cell types during the regeneration of the zebrafish caudal fin. Nuclei were isolated from caudal fin tissues at both pre-injury and various stages of regeneration (1, 2, 4, and 6 days post-amputation, dpa) for single-nucleus multi-omics sequencing (paired snATAC-seq and snRNA-seq).

本研究旨在勾勒斑马鱼尾鳍再生过程中不同细胞类型的转录组学全景与染色质可及性图谱。研究人员从损伤前及截肢后各再生阶段——即截肢后1、2、4、6天（days post-amputation，简称dpa）——的尾鳍组织中分离细胞核，开展单细胞核多组学测序，包含配对的单细胞核转座酶可及性染色质测序（snATAC-seq）与单细胞核RNA测序（snRNA-seq）。