Impact of marine heatwaves on diploid and triploid Pacific Oyster gene expression

Adult diploid and triploid Pacific oysters were exposed to environmental conditions present during marine heatwaves. Treatments included elevated seawater temperature (single stressor; 30C), elevated seawater temperature followed by aerial emersion (multiple stressor; 30C + 44C for 6h), and a control (20C, submerged). RNA was extracted from flash frozen ctenidium (gill) tissue samples and sequenced using the QuantSeq 3-prime mRNA-Seq (Tag-seq) protocol (v.015UG009V0251, Lexogen, Vienna, Austria).

本研究将成年二倍体与三倍体太平洋牡蛎暴露于海洋热浪时期的环境条件中。实验处理设置如下：单一胁迫因子组（升高海水温度，30℃）、多重胁迫因子组（先升高海水温度至30℃，随后进行44℃空气暴露，持续6小时），以及对照组（20℃，浸没于海水中）。从快速冷冻的栉鳃（ctenidium，即鳃）组织样本中提取总RNA，并采用QuantSeq 3'端mRNA测序（Tag-seq）方案（版本号v.015UG009V0251，奥地利维也纳Lexogen公司）完成测序。