LC-AC-95 Specialized Pro-resolving Mediators in Bovine Plasma and Adipocytes: LPS Single Infusion Raw Data

We characterized the impact of bacterial lipopolysaccharide (LPS) administration on the in vivo plasma oxylipin profile in dairy cattle and in vitro cultured bovine adipocytes. For in vivo experiments, cows received either sterile saline (SAL) or saline containing LPS at 1 ug/kg bodyweight infused into the jugular vein over 20 min. Plasma samples were collected immediately before infusions (PRE), 2 h after (+2H), and 12 h after (+12H) and flash-frozen. For in vitro experiments, mature bovine adipocytes were exposed to 1 ug/mL LPS for 3 h. Following, culture medium was collected and flash-frozen. Oxylipin metabolites in plasma and adipocyte culture medium were then quantified via LC-MS/MS.

本研究表征了细菌脂多糖（lipopolysaccharide, LPS）给药对奶牛体内血浆氧脂素谱及体外培养牛脂肪细胞的影响。体内实验中，奶牛分别接受无菌生理盐水（SAL）处理，或经颈静脉持续20分钟输注剂量为1 μg/kg体重的含LPS生理盐水；分别于给药前（PRE）、给药后2小时（+2H）及给药后12小时（+12H）采集血浆样本并快速冷冻保存。体外实验中，将成熟牛脂肪细胞以1 μg/mL浓度的LPS处理3小时，随后收集培养液并快速冷冻保存；最终采用液相色谱-串联质谱（LC-MS/MS）对血浆及脂肪细胞培养液中的氧脂素代谢物进行定量分析。