The de novo synthesis of GABA and its gene regulatory function are indispensable for HCC metastasis. The de novo synthesis of GABA and its gene regulatory function are indispensable for HCC metastasis

To investigate the mechanism of GABA in the regulation of liver cancer metastasis, we treated Huh7 cell lines with or without GABA. We then performed gene expression profiling analysis using data obtained from RNA-seq. Overall design: Comparative gene expression profiling analysis of RNA-seq data for Huh7 cells with or without GABA treatment for 24h.

为探究γ-氨基丁酸（GABA）调控肝癌转移的分子机制，我们对Huh7细胞系分别施加γ-氨基丁酸处理与不予处理。随后基于RNA测序（RNA-seq）获取的实验数据开展基因表达谱分析。整体实验设计：针对经24小时γ-氨基丁酸处理与未处理的Huh7细胞的RNA测序数据，进行对比性基因表达谱分析。