兰科植物菌根真菌目标基因的表达分析与共生机制解析

基于转录组数据和 qRT‐PCR 对铁皮石斛根茎叶组织、共生与非共生生长的种子和根、冷诱导和茉莉酸甲酯诱导后叶片中的 STR 家族基因的表达情况进行检测和分析，从铁皮石斛基因组中预测获得 10 个 STR 成员，具有典型的“Str_synth”结构域，且启动子区存在大量顺式作用元件，涉及到茉莉酸甲酯响应和低温响应等多个生物学过程；DoSTRs 基因家族不同成员表达模式存在较大差异，DoSTR3、DoSTR7、DoSTR10 在叶片中的表达量高于茎和根中，DoSTR9 和 DoSTR2 在铁皮石斛种子萌发时显著高表达，DoSTR5 和 DoSTR10 在茉莉酸甲酯处理早期，具有明显的上调表达趋势，推测 STR 家族可能参与不同的生物学过程。

Based on transcriptome data and qRT-PCR, we detected and analyzed the expression profiles of STR family genes in root, stem and leaf tissues of Dendrobium officinale, seeds and roots grown under symbiotic vs. non-symbiotic conditions, and leaves treated with cold induction or methyl jasmonate (MeJA). Ten STR members were predicted from the D. officinale genome, all containing the typical 'Str_synth' domain; their promoter regions harbor numerous cis-acting elements involved in multiple biological processes including MeJA response and low-temperature response. There are significant differences in expression patterns among different members of the DoSTRs gene family: DoSTR3, DoSTR7 and DoSTR10 show higher expression levels in leaves than in stems and roots; DoSTR9 and DoSTR2 are significantly upregulated during seed germination of D. officinale; DoSTR5 and DoSTR10 exhibit a clear upward expression trend in the early stage of MeJA treatment. It is speculated that the STR family may participate in diverse biological processes.